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喜訊:恭賀南京農(nóng)業(yè)大學(xué)王老師成功領(lǐng)取獎學(xué)金

閱讀次數(shù):1204    發(fā)布時間:2021/5/11 10:37:43

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五月,大家的論文寫的怎么樣了?
首先我們要恭賀一下來自南京農(nóng)業(yè)大學(xué)的王老師,2021年王老師在《International Journal of Nanomedicine》(國際納米醫(yī)學(xué)雜志)發(fā)表了SCI文獻,發(fā)表的文獻影響因子(IF)達到了5.15分!然后讓我們恭喜王老師領(lǐng)到了恒遠(yuǎn)生物的獎學(xué)金!
 
這篇論文寫的非常棒,其中的很多實驗方法和思路值得我們閱讀,小編想要分享給大家,希望能夠?qū)ψ鰧嶒灥男』锇閭冇幸稽c點幫助,也希望大家積極的參與我們的“發(fā)論文贏獎學(xué)金”活動,小編等著你們的投稿哦,下一個領(lǐng)獎學(xué)金的說不定就是你!
 
一、恒遠(yuǎn)產(chǎn)品文獻分享:
 
【文獻標(biāo)題】
Nanoparticles of Chitosan/Poly(D,L-Lactide-CoGlycolide) Enhanced the Immune Responses of Haemonchus contortus HCA59 Antigen in Model Mice
【作者單位】南京農(nóng)業(yè)大學(xué)(Nanjing Agricultural University)
【文獻中引用產(chǎn)品】
小鼠免疫球蛋白G1(IgG1)ELISA試劑盒
小鼠IgG2a抗體亞型ELISA試劑盒
 
小鼠免疫球蛋白M(IgM)ELISA試劑盒
 
小鼠白介素4(IL-4)ELISA試劑盒
 
小鼠白介素12(IL-12)ELISA試劑盒
 
小鼠白介素17A(IL-17A)ELISA試劑盒
 
小鼠γ干擾素(IFN-γ)ELISA試劑盒
 
小鼠轉(zhuǎn)化生長因子β(TGF-β) ELISA試劑盒
 
【關(guān)鍵詞】
【DOI】https://doi.org/10.2147/IJN.S301851 
【影響因子(IF)】5.15
【出版期刊】《International Journal of Nanomedicine》
 
二、產(chǎn)品原文引用
 
Cytokine Assays
 
The levels of IL-4, IL-12, IL-17, IFN-γ, and TGF-β in sera from the ten groups were investigated with commercial ELISA kits (HengYuan, Shanghai, China) according to the manufacturer’s guidelines.
 
 
Characterization of rHCA59-CS NPs and rHCA59-PLGA NPs

After conjugation, PLGA and Chitosan nanoparticles were precipitated and the free amounts of rHCA59 protein were determined by a BCA protein assay kit. It was found that 89% and 71% of rHCA59 were conjugated to PLGA and Chitosan, respectively. In other words, a total of 1.78 mg of protein bound to PLGA, and 1.42 mg of protein bound to Chitosan.A scanning electron microscopy was used to identify the size and morphology of PLGA and Chitosan nanoparticles. The SEM results showed that both PLGA and Chitosan nanoparticles’ appearances were smooth. The size of rHCA59-PLGA NPs was from 180 nm to 402 nm (Figure 1A) and the size of rHCA59-CS NPs was from 200 nm to 500 nm (Figure 1B).


 
 
Figure 3 Effects of different antigen delivery systems on the expressions of multiple cytokines. Sera were collected and detected on day 14 by ELISA. 
 

Notes: (1): PBS group (blank control). (2): pET-32a protein group. (3): PBS-PLGA group. (4): PBS-CS group. (5): PBS-CFA group. (6): rHCA59 group. (7): rHCA59-PLGA group. (8): rHCA59-CS group. (9): rHCA59-CFA group. (10): rHCA59-CS-PLGA group. (A) IL-12. (B) IL-17. (C) IFN-γ. (D) IL-4. (E) TGF-β. Data are representative of triplicate independent experiments (*p < 0.05, **p < 0.01, and ***p < 0.001). Abbreviations: PLGA, poly-D,L-lactide-co-glycolide; CS, Chitosan; CFA, complete Freund’s adjuvant; IL, interleukin; ELISA, enzyme linked immunosorbent assay


 
 
Figure 5 Effects of different antigen delivery systems on T lymphocyte subsets.

Notes: rHCA59 loaded nanoparticles could affect the proportions of CD4+ T cells and CD8+ T cells in immunized mice. The percentages of CD4+ T cells (A1–A10) and CD8+ T cells (B1–B10) in ten groups were investigated by flow cytometry on day 14 after immunization. (C and D) Different treatments affected the proportions of CD4+ T cells and CD8+ T cells. (A1 and B1) PBS group (blank control). (A2 and B2) PBS-CFA group. (A3 and B3) PBS-PLGA group. (A4 and B4) PBS-CS group. (A5 and B5) pET-32a protein group. (A6 and B6) rHCA59 group. (A7 and B7) rHCA59-CFA group. (A8 and B8) rHCA59-CS group. (A9 and B9) rHCA59-PLGA group. (A10 and B10) rHCA59-CS-PLGA group. The results shown here are from three independent experiments (*p < 0.05, **p < 0.01, and ***p < 0.001). Abbreviations: CD4, cluster of differentiation 4; CD8, cluster of differentiation 8; T cells, thymus cells; PLGA, poly-D,L-lactide-co-glycolide; CS, Chitosan; CFA, complete Freund’s adjuvant.



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